Fluorescence Excitation–Emission Matrix (EEM) Spectroscopy for Rapid Identification and Quality Evaluation of Cell Culture Media Components
نویسندگان
چکیده
منابع مشابه
Rapid quantification of tryptophan and tyrosine in chemically defined cell culture media using fluorescence spectroscopy.
The rapid and inexpensive analysis of the complex cell culture media used in industrial mammalian cell culture is required for quality and variance monitoring. Excitation-emission matrix (EEM) spectroscopy combined with multi-way chemometrics is a robust methodology applicable for the analysis of raw materials, media, and bioprocess broths. We have shown that the methodology can identify compos...
متن کاملeffect of seed priming and irrigation regimes on yield,yield components and quality of safflowers cultivars
این مطالعه در سال 1386-87 در آزمایشگاه و مزرعه پژوهشی دانشگاه صنعتی اصفهان به منظور تعیین مناسب ترین تیمار بذری و ارزیابی اثر پرایمینگ بر روی سه رقم گلرنگ تحت سه رژیم آبیاری انجام گرفت. برخی از مطالعات اثرات سودمند پرایمینگ بذر را بر روی گیاهان مختلف بررسی کرده اند اما در حال حاضر اطلاعات کمی در مورد خصوصیات مربوط به جوانه زنی، مراحل نموی، عملکرد و خصوصیات کمی و کیفی بذور تیمار شده ژنوتیپ های م...
Prediction of cell culture media performance using fluorescence spectroscopy.
Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in efficient manufacturing. We are exploring the use of rapid "holistic" analytical methods that can be used for routine screening of cell culture media used in industrial biotechno...
متن کاملRapid Characterisation and Quality Control of Complex Cell Culture Media Solutions using Raman Spectroscopy
The use of Raman spectroscopy coupled with chemometrics for the rapid identification, characterisation, and quality assessment of complex cell culture media components used for industrial mammalian cell culture was investigated. Raman spectroscopy offers significant advantages for the analysis of complex, aqueous based materials used in biotechnology because there is no need for sample preparat...
متن کاملEvaluation of Culture Media for the Rapid Isolation of Dermatophytes
Dermatophytes are prevalent causes of cutaneous mycoses and, unlike many other fungal pathogens, are able to cause disease in immunocompetent individuals. They infect keratinized tissue such as skin, hair, and nails, resulting in tinea infections, including ringworm. The dermatophytes include three genera of molds in the class Euascomycetes: Trichophyton, Microsporum, andEpidermophyton. Dermato...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Spectroscopy
سال: 2011
ISSN: 0003-7028,0003-7028
DOI: 10.1366/11-06383